Lithium Borate

Lithium Borate can be used in the laboratory as buffer for gel electrophoresis of DNA and RNA. It has a lower conductivity, produces crisper resolution, and can be run at higher speeds than can gels made from TBE or TAE (5-50V/cm as compared to 5-10V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TBE/TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected with lithium boric acid gels. Lithium borate is also an ingredient for use in making glasses and ceramics.

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Lithium Borate

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